Bioassay and molecular detection of insecticides resistance of Aedes aegypti, vector of dengue in Central Java Province, Indonesia

Abstract. Sayono, Nurullita U, Handoyo W, Tyasningrum WS, Chakim I, Budiharjo A. 2023. Bioassay and molecular detectionsof insecticides resistance of Aedes aegypti, vector dengue in Central Java Province, Indonesia. Biodiversitas 24: 300-307. The emergence insecticide-resistant strains among aegyptipopulations hampered Dengue control programs the endemic areas. Moreover, to understand current situation distribution insecticide status Ae. aegypti cypermethrin, malathion, temephos compounds, we conducted morphological detection areas Mosquito larvae were obtained from thirteen villages five representing different altitudes. Larval adult stage colony each village was subjected a bioassay test based on WHO procedures. Subsequently, they sampled analysis identify 1016G kdr allele using allele-specific polymerase chain reaction (AS-PCR). Mortality after exposure ranged 16-86%, 75-100%, 6-51%, respectively. These findings showed that populations resistant cypermethrin temephos, although malathion-susceptible found 23.08% altitudinal localities. result AS-PCR indicated homozygous (G/G) heterozygous (V/G) alleles codon 1016 AaNav gene throughout study site development multiple use ofcypermethrin compounds must be delayed for at least years, while malathion can still used selectively population several areas, namely Karangjati, Gebugan (Semarang District), Rowosari Semarang City.